Invitrogen™

E-Gel™ Agarose Gels with SYBR™ Safe DNA Gel Stain, 4%

Catalog number:  A45206

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Description

Invitrogen E-Gel precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis.Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or DNA fragment libraries.

Features of E-Gel agarose gels include:
Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
In-process control—view DNA sample migration in real time
Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.

Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.

Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.

Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.

Learn more about the E-Gel Power Snap Electrophoresis System ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Percentage: 4 %
Gel Type: E-Gel™
In-Gel Dye: SYBR™ Safe
Product Line: E-Gel™
Product Size: 2 x 10 gels
Wells: 11 wells
Shipping Condition: Room Temperature

Contents & storage

Each box contains 10 gels. Store strictly at room temperature. Do not feeze and avoid cold spots near freezers or other uncontrolled environments.

Documents

Manuals & protocols