Ion AmpliSeq™ Mouse BCR IGH SR Assay, RNA - FAQs

The assay provides sufficient reagents for rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA consists of the Ion AmpliSeq Mouse BCR IGH-SR Panel, the Ion AmpliSeq Library Kit Plus (Cat. No. 4488990), and the Ion Torrent NGS Reverse Transcription Kit (Cat. No. A45003). Sufficient reagents are provided for the rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The assay input requirement ranges from 25 ng to 1 µg.

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This assay is compatible with isolated B cells, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh frozen tissue samples, including FFPE and bone marrow samples.

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The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA (Cat. No. A45487) is a highly sensitive RNA-based NGS assay that enables the characterization of the immunoglobulin heavy chain complementarity-determining region 3 (CDR3) sequences. The assay accurately measures B cell repertoire diversity and clonal expansion in a wide variety of mouse sample types.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.