Oncomine™ Comprehensive Assay Plus, manual library preparation - FAQs

We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is designed to prepare barcoded sample libraries from DNA and RNA. The kit consists of Oncomine Comprehensive Assay Plus DNA (2-pool) (Part No. A45615), RNA Oncomine Comprehensive Assay v3M (2-pool) (Part No. A33637), and two kits of Ion AmpliSeq Library Kit Plus (Cat. No. 4488990). Sufficient reagents are provided to prepare libraries from 24 samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

10 ng of nucleic acid input per pool (20 ng of DNA and 20 ng of RNA) isolated from FFPE samples, including fine needle biopsies, is needed for the Oncomine Comprehensive Assay Plus, manual library preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The assay is optimized for use with formalin-fixed paraffin embedded (FFPE) samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is a targeted, next-generation sequencing (NGS) assay that enables the detection of single nucleotide variants (SNVs), insertions and deletions (INDELs), copy number variations (CNVs), gene fusions, splice variants, tumor mutational burden (TMB), and microsatellite instability (MSI) across 500+ unique genes. This version of the assay is designed for manual library preparation, not for automated library preparation using the Ion Chef System.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.