Ion AmpliSeq™ Microbiome Health Research Assay GX - FAQs

Genexus Software version 6.8 is needed to run the Ion AmpliSeq Microbiome Health Research Assay GX kit.

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We recommend using the Qubit dsDNA Quantification Assay Kits (Cat. No. Q32850, Q32851, Q32853, Q32854).

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We recommend using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes (Cat. No. A42358) or MagMAX Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate (Cat. No. A42357) for isolating bacterial DNA from research stool samples.

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No, only the GX7 Chip has been tested with the Ion AmpliSeq Microbiome Health Research Assay GX kit.

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The Ion AmpliSeq Microbiome Health Research Assay GX kit can detect 73 species of bacteria.

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24 samples (12 samples/lane) can be run with the Ion AmpliSeq Microbiome Health Research Assay GX kit.

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The Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) can be used with Ion AmpliSeq or Ion AmpliSeq HD libraries.

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No, the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) should be used with the GX7 Chip. Each kit allows for the automated template preparation and chip loading of two GX7 chips using the Genexus Integrated Sequencer.

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The Genexus Strip 3-GX7 should be stored at 2-8 degrees C and Genexus Strip 4 should be stored at -30 to -10 degrees C.

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The Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) consists of 8 pairs of 3-GX7 and 4 strips, allowing for up to 2 sequencing runs per kit.

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The GX7 Chip and Genexus Coupler (Cat. No. A46731) expands the capabilities of the Genexus system by generating more reads per lane than the GX5 Chip, increasing the throughput of custom Ion AmpliSeq assays and allowing the use of the Ion AmpliSeq Microbiome Health Research Assay GX.

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The GX7 Chip is compatible with the Genexus Software version 6.8.0 and later.

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The Genexus Coupler should not be reused. The chip and coupler should be changed together.

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The GX7 Chip and Genexus Coupler (Cat. No. A46731) can remain installed on the instrument until all lanes are used up, or within two weeks of the initial run, whichever comes first.

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We recommend storing GX7 Chip and Genexus Coupler (Cat. No. A46731) at room temperature.

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The GX7 Chip and Genexus Coupler (Cat. No. A46731) consists of two GX7 chips and two Genexus couplers, sufficient for up to 8 sequencing runs or two initializations of the Genexus Integrated Sequencer.

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The GX7 chip provides higher throughput compared to the GX5 chip. The GX5 chip runs 12-15 million reads per lane whereas the GX7 chip runs 20-25 million reads per lane.

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The GX7 Chip generates 20-25 million sequencing reads per lane for the 200 bp library, or 80-100 million reads per chip.

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You need Genexus Software v6.8 or later in order to run GX7 chips.

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