RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit - FAQs

Q: Which total RNA isolation methods can I use with RiboMinus Bacteria 2.0 Transcriptome Isolation Kit?

We recommend isolating total RNA using one of the following methods: - TRIzol Plus RNA Purification Kit (Cat. No. 12183555) - PureLink RNA Mini Kit (Cat. No. 12183018A, 12183020, 12183025) - MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828).

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5 product FAQs found

What are the shipping conditions and stability of the RiboMinus Pan-Prokaryote Probe Mix?

The RiboMinus Pan-Prokaryote Probe Mix is a DNA probe and is shipped on dry ice (while the rest of the kit is shipped at 4 degrees C). The RiboMinus Pan-Prokaryote Probe Mix is stable for at least a year when stored at -20 degrees C.

Which Pan-Prokaryote species are compatible with RiboMinus Bacteria 2.0 Transcriptome Isolation Kit?

For species compatibility inquiries (not already listed on the product page), please send an email to techsupport@thermofisher.com and provide a copy of your species rRNA FASTA sequences.

How much RNA can I expect to recover after rRNA depletion using RiboMinus Bacteria 2.0 Transcriptome Isolation Kit?

We typically recover approximately 3-6% of input total RNA following the rRNA depletion, concentration, and purification steps.

What are the total RNA input amounts and RiboMinus Pan-Prokaryote Probe Mix volumes I should use in the RiboMinus Bacteria 2.0 Transcriptome Isolation Kit workflow?

Use 1 µL (100 pmoles) RiboMinus Pan-Prokaryote Probe Mix with 100 ng to < 1 µg total RNA sample input. Use 3 µL (300 pmoles) RiboMinus Pan-Prokaryote Probe Mix with 1 µg to 5 µg total RNA sample input.

Which total RNA isolation methods can I use with RiboMinus Bacteria 2.0 Transcriptome Isolation Kit?

We recommend isolating total RNA using one of the following methods: - TRIzol Plus RNA Purification Kit (Cat. No. 12183555) - PureLink RNA Mini Kit (Cat. No. 12183018A, 12183020, 12183025) - MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828).