Thermo Scientific™

POROS™ Oligo (dT)25 Affinity Resin

Catalog number: A47384
Thermo Scientific™

POROS™ Oligo (dT)25 Affinity Resin

Catalog number: A47384
Catalog Number
A47384
Unit Size
1,000 mL
Price (USD)
Availability
-
Quantity
-
Catalog Number
Unit Size
10 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
25 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
50 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
250 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
5,000 mL
Price (USD)
Availability
-
Quantity
-
Catalog Number
Unit Size
10,000 mL
Price (USD)
Availability
-
Quantity
-
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A473841,000 mLContact Us ›--
A4860510 mL
-
A4860625 mL
-
A4738250 mL
-
A47383250 mL
-
A473855,000 mLContact Us ›--
A4738610,000 mLContact Us ›--
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

POROS Oligo (dT)25 Affinity Resin is designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. The resin effectively separates mRNA from components of the transcription reaction process, such as enzymes and plasmid DNA.

POROS Oligo (dT)25 Affinity Resin was developed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. Unlike alternate approaches, the resin selectively captures the mRNA via the polyadenylated (polyA) tail using simple salt and water purification steps.

Features of POROS Oligo (dT)25 Affinity Resin include:

  • Easy mRNA purification from crude transcription mix to effectively remove plasmid DNA and other IVT components
  • Simplified workflow helps to maximize efficiency, thereby reducing complexity of subsequent polish steps
  • Excellent scalability
  • Non-animal derived

POROS Oligo (dT)25 Affinity Resin allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.

Pack sizes of 1 L and above come with a regulatory support package. Worldwide technical support is also available.

Main characteristics

  • Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
  • Average particle size: 50 μm
  • Ligand: poly(dT) 25mer with proprietary linker
  • Binding capacity: >2 mg mRNA per mL of resin
  • Binding conditions: 0.5–1 M salt, common buffers, neutral pH
  • Elution conditions: Low salt, common buffers, neutral pH
  • Mechanical resistance: 100 bar (1450 psi; 10 MPa)
  • Thermal stability: allows sample denaturing at 65°C if needed
  • Formulation buffer: 18% (v/v) ethanol

Oligo (dT) ligands are manufactured using a synthetic manufacturing process that are free of animal components.

Specifications

Binding Capacity
>2 mg mRNA per mL of resin
Matrix
Cross-linked poly(styrene-divinylbenzene) POROS beads
Shipping Condition
Wet Ice
Particle Size
50 μm
Form
Liquid Suspension
Product Line
POROS™
Quantity
1 L
Target
mRNA
Type
Bulk Resin
Stationary Phase
Poly(dT) 25mer with proprietary linker

Contents & Storage

Store at 2°C to 8°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text