Thermo Scientific™

Pierce™ HeLa Digest/PRTC Standard

Catalog number: A47996
Thermo Scientific™

Pierce™ HeLa Digest/PRTC Standard

Catalog number: A47996
Catalog Number
A47996
Unit Size
10 µg
Price (USD)
Price: 164.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 10 µg
Price (USD)
Price: 640.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A4799610 µg
Price: 164.00
Your Price:
***
A479975 x 10 µg
Price: 640.00
Your Price:
***
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The Thermo Scientific Pierce HeLa Digest/PRTC Standard is a validated complex mammalian protein digest containing additional PRTC heavy isotope-labeled peptides designed for LC/MS performance testing.

Features of HeLa Digest/PRTC Standard include:
Convenient—pre-mixed formulation of HeLa digest and PRTC standard in a ready-to-use format
Informative—enables robust performance assessment of LC/MS instruments
Verified—high-quality, consistent mixture documented via lot-specific certificates of analysis
Stable—provided in a lyophilized format

The Pierce HeLa Digest/PRTC Standard is a lyophilized mixture of 10 µg of HeLa protein digest with 5 pmol PRTC peptides to assess chromatography MS instrument performance. The protein digest is derived from a well-established adenocarcinoma reference HeLa S3 cell line that expresses over 15,000 proteins with relevant post-translational modifications. This complex digest is an ideal standard for complex proteome mass spectrometry applications.

The included Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy isotope-labeled peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HFs) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time. In combination, the mixture is a powerful tool to optimize and assess LC parameters, identify the total peptide elution window, and optimize and monitor MS instrumentation.

This standard is specifically formulated for LC/MS experiments and does not contain salts or detergents. Using the standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.

Applications:
• Qualitative LC assessment
• QC assay tool for assessment of LC/MS system performance
• LC/MS standardization
• LC/MS method development and optimization
• Normalize results for variation in retention times and peak intensities between runs

WARNING: Cancer – www.P65Warnings.ca.gov

Specifications

Chemical Name or Material
HeLa Digest/PRTC Standard
Synonym
HeLa/PRTC
Grade
MS-grade
Packaging
Lyophilized in glass vial
Quantity
10 µg

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