These RoboColumns are pre-packed with POROS Oligo (dT)25 Affinity Resin, designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. The resin effectively separates mRNA from components of the transcription reaction process, such as enzymes and plasmid DNA.
RoboColumn products are small chromatography columns that are provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.POROS Oligo (dT)25 Affinity Resin
was developed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. Unlike alternate approaches, the resin selectively captures the mRNA via the polyadenylated (polyA) tail using simple salt and water purification steps.
Features of POROS Oligo (dT)25 Affinity Resin include:
• Easy mRNA purification from crude transcription mix to effectively remove plasmid DNA and other IVT components
• Simplified workflow helps to maximize efficiency, thereby reducing complexity of subsequent polish steps
• Excellent scalability
• Non-animal derived
POROS Oligo (dT)25 Affinity Resin allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.
Oligo (dT) ligands are manufactured using a synthetic manufacturing process that are free of animal components.
Other POROS ion exchange resins, as well HIC and affinity resins, are also available in this RoboColumn format. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.
RoboColumn is a registered trademark of ATOLL GmbH.