Thermo Scientific™

VitroEase™ Buffer Screening Kit and Detergents

Catalog number: A49856
Thermo Scientific™

VitroEase™ Buffer Screening Kit and Detergents

Catalog number: A49856
The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis.
 
Catalog Number
A49856
Unit Size
1 kit
Description
VitroEase™ Buffer Screening Kit
Price (USD)
Availability
-
Full specifications
Quantity
ColorColorless
DescriptionVitroEase™ Buffer Screening Kit
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
QuantityBuffers: 400 μL, Detergents: 100 μL
PackagingPolypropylene Vials
FormLiquid
Unit Size1 kit
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent A (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration0.3% (10X CMC)
DescriptionDetergent A (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferCTAB
Unit Size2 x 100 μL
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent B (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration4.9% (10X CMC)
DescriptionDetergent B (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferCHAPS
Unit Size2 x 100 μL
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent C (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration2.7% (10X CMC)
DescriptionDetergent C (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferOG
Unit Size2 x 100 μL
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent D (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration0.1% (10X CMC)
DescriptionDetergent D (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferTween™-20
Unit Size2 x 100 μL
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent E (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration1% (10X CMC)
DescriptionDetergent E (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferDM
Unit Size2 x 100 μL
Catalog Number
Unit Size
2 x 100 μL
Description
Detergent F (refill)
Price (USD)
Availability
-
Full specifications
Quantity
Concentration0.7% (10X CMC)
DescriptionDetergent F (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferFOM
Unit Size2 x 100 μL
Showing 7 of 7
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
A49856Full specifications
1 kitVitroEase™ Buffer Screening Kit
-
ColorColorless
DescriptionVitroEase™ Buffer Screening Kit
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
QuantityBuffers: 400 μL, Detergents: 100 μL
PackagingPolypropylene Vials
FormLiquid
Unit Size1 kit
A65372Full specifications
2 x 100 μLDetergent A (refill)
-
Concentration0.3% (10X CMC)
DescriptionDetergent A (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferCTAB
Unit Size2 x 100 μL
A65373Full specifications
2 x 100 μLDetergent B (refill)
-
Concentration4.9% (10X CMC)
DescriptionDetergent B (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferCHAPS
Unit Size2 x 100 μL
A65374Full specifications
2 x 100 μLDetergent C (refill)
-
Concentration2.7% (10X CMC)
DescriptionDetergent C (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferOG
Unit Size2 x 100 μL
A65375Full specifications
2 x 100 μLDetergent D (refill)
-
Concentration0.1% (10X CMC)
DescriptionDetergent D (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferTween™-20
Unit Size2 x 100 μL
A65376Full specifications
2 x 100 μLDetergent E (refill)
-
Concentration1% (10X CMC)
DescriptionDetergent E (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferDM
Unit Size2 x 100 μL
A65377Full specifications
2 x 100 μLDetergent F (refill)
-
Concentration0.7% (10X CMC)
DescriptionDetergent F (refill)
For Use With (Equipment)Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Quantity2 x 100 μL
PackagingPolypropylene Vials
FormLiquid
For Use With (Application)Cryo-EM
BufferFOM
Unit Size2 x 100 μL
Showing 7 of 7

The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis.

Features of the VitroEase Buffer Screening Kit include:
Ease of use—14 buffers and 6 detergents pre-formulated at 10X concentration ready to be spiked in with your protein sample with minimal sample dilution
Convenient—buffers and detergents provided with color-coded caps that correlate with color-coded screening strategy
Comprehensive—optimized screening strategy encompassing two rounds of screening with 24 grids with trouble-shooting suggestions
Flexible—wide range of common buffers and detergents for cryo-EM sample preparation sufficient for sample optimization of 400 grids
Validated—components and screening strategy validated with single particle cryo-EM analysis

Cryo-EM sample screening evaluates protein behavior in various buffers and additive conditions with the end goal of identifying the optimal conditions in which the protein is stable, randomly oriented, and equally distributed in ice. Kit buffers are provided with pHs from 3.6 to 8.9 pre-mixed with various salts and divalent cations. Detergents are provided at 10X concentrations with an easy-to-use table to convert volume to CMC concentration.

The comprehensive sample screening strategy uses two rounds of screening and 24 grids, taking approximately two days to process (figure below). In the first round, optimal protein concentration, buffer, and pH conditions are determined. In the second round, detergents are further optimized. A condensed strategy is also provided using 8 grids and taking one day to process, which can be used when little sample is available or for use with side entry or semi-automated sample loading systems, like the Tundra Cryo-TEM.

The recommended screening strategies are designed for use with the Thermo Scientific Vitrobot Mk4 and Krios, Glacios, Arctica microscopes with Autoloader systems and EPU multigrid.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only.

Contents & Storage

• Buffer 1 (10X): C2H3O2 (0.5 M), Cl (1.5 M); pH 3.6 400 μL
• Buffer 2 (10X): C2H3O2 (0.5 M), KCl (3 M); pH 3.6 400 μL
• Buffer 3 (10X): MES (0.5 M), Cl (1.5 M); pH 5.5 400 μL
• Buffer 4 (10X): MES (0.5 M), KCl (3 M); pH 5.5 400 μL
• Buffer 5 (10X): Tris-HCl (0.5 M), Mg (CH2COO)2 (0.1 M), Cl (1.5 M); pH 7.2 400 μL
• Buffer 6 (10X): Tris-HCl (0.5 M), MgCl2 (0.1 M), CH3CO2K (1.5 M); pH 7.2 400 μL
• Buffer 7 (10X): Tris-HCl (0.5 M), Mg(CH3COO)2 (0.1 M), KCl (3 M); pH 7.2 400 μL
• Buffer 8 (10X): HEPES (0.5 M), Cl (1.5 M); pH 7.4 400 μL
• Buffer 9 (10X): HEPES (0.5 M), KCl (3 M); pH 7.4 400 μL
• Buffer 10 (10X): HEPES (0.5 M), Mg(CH3COO)2 (50 mM), CH3CO2K (1.5 M); pH 7.4 400 μL
• Buffer 11 (10X): HEPES (0.5 M), MgCl2 (50 mM), CaCl2 (50 mM), Cl (1.5 M); pH 7.4 400 μL
• Buffer 12 (10X): PBS (1.37 M Cl, 270 mM KCl, 43 mM 2 HPO); pH 7.4 400 μL
• Buffer 13 (10X): Bicine buffer (0.5 M), Cl (1.5 M); pH 8.5 400 μL
• Buffer 14 (10X): CAPSO (0.5 M), KCl (3 M); pH 8.9 400 μL
• Detergent A (10X): CTAB (0.3%) 100 μL
• Detergent B (10X): CHAPS (4.9%) 100 μL
• Detergent C (10X): OG (2.7%) 100 μL
• Detergent D (10X): Tween™-20 Detergent (0.1%) 100 μL
• Detergent E (10X): DM (1%) 100 μL
• Detergent F (10X): FOM (0.7%) 100 μL

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