Thermo Scientific™

Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible

Catalog number: A50585
Thermo Scientific™

Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible

Catalog number: A50585
Catalog Number
A50585
Unit Size
50 mL
Price (USD)
582.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
144.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
250 mL
Price (USD)
2,515.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
4,500.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A5058550 mL
582.00
Your Price:
***
A5058410 mL
144.00
Your Price:
***
A50586250 mL
2,515.00
Your Price:
***
A50587500 mL
4,500.00
Your Price:
***
Product Overview
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Pierce high-capacity EDTA-compatible Ni-IMAC resin is designed for the purification of over-expressed His-tagged proteins from cell culture media. The matrix is a 6% crosslinked agarose with optimal properties for batch, gravity column, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC resin include:
• Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
• Reusable—can be regenerated up to 10 times using the CIP protocol

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping off the metal. Because of its chelator stability characteristic (EDTA tolerant), Pierce high-capacity EDTA-compatible Ni-IMAC resin is especially suited for purifying expressed His-tagged proteins that are secreted into cell culture media or for purifying intracellular His-tagged proteins that need the presence of EDTA to maintain stability and function. In both cases, large quantities of samples can be purified without the Ni metal stripping off of the support, thereby providing consistent high capacity yields.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
50 mL
Column Type
Bottle
Form
Liquid Suspension
Product Line
Pierce™
Type
Resin
Binding Property
Chelator Stable
Description
EDTA compatible Ni-IMAC resin for His-tag protein purification
Matrix
6% crosslinked agarose
Shipping Condition
Wet ice

Contents & Storage

50 mL settled resin, supplied as 100 mL 50% slurry. Store at 2–8°C.

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