Invitrogen SuperScript IV RT-LAMP Master Mix is a solution designed for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

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Features of SuperScript IV RT-LAMP Master Mix include:
• Fast RNA target detection using evolved Bst DNA polymerase optimized for isothermal amplification in as little as 5 minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity using optimized buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection; includes SYTO 9 dye for real-time detection

SuperScript IV RT-LAMP Master Mix is designed for fast viral pathogen RNA amplification, providing high detection sensitivity, specificity, and robust performance. The optimized master mix utilizes best-in-class enzymes that enable reliable target detection in 5–15 minutes for a variety of viral RNA targets and concentrations.

SuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with high inhibitor tolerance and rapid target amplification at a temperature optimum of 65°C, as well as SuperScript IV Reverse Transcriptase with increased thermostability, speed, and inhibitor tolerance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.

SuperScript IV RT-LAMP Master Mix performance has been validated for multiple viral RNAs, including measles, influenza, and SARS-CoV-2 RNA, using multiple primer sets and purified RNA samples. Furthermore, the master mix can be used to detect DNA with a reaction speed similar to or faster than that for RNA targets.

SuperScript IV RT-LAMP Master Mix is available in a convenient single-tube format with SYTO 9 dye for real-time detection. The master mix format offers flexibility to select and test different primers for desired RNA targets and to use with end-point or real-time detection methods.