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TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX), 50 mg - FAQs

TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX), 50 mg - FAQs

View additional product information for Mass Spectrometry Compatible Crosslinkers - FAQs (A52287, A52286)

3 product FAQs found

What is the advantage of using phospho-enrichable crosslinkers?

Phospho-enrichable crosslinkers such as DSPP (Disuccinimidyl Phenyl Phosphonic Acid, PhoX) and TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX) are great tools for studying protein-protein Interaction, protein 3D structure, and for mass spectrometry (MS) analysis. They can be used to enrich low-abundance crosslinked peptides and thereby improve MS identification rates.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX) cell-permeable?

Yes, TBDSPP is cell-permeable. After the crosslinking reaction and before the phospho-enrichment step, TBDSPP needs to be de-protected.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is DSPP (Disuccinimidyl Phenyl Phosphonic Acid, PhoX) cell-permeable?

DSPP is not cell-permeable. We offer TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX), Cat. No. A52287 that is a cell-permeable phopho-enrichable crosslinker.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.