Efficient-Pro™ Medium, 1000 mL - FAQs

The shelf-life of Efficient-Pro Medium is 12 months from the date of manufacture. The expiration date is provided on the product label.

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Yes, however, we recommend reducing the L-glutamine concentration if a decrease in cell viability is observed. The recommended glutamine concentration is 2-8 mM. Another way to reduced ammonia production is to use GlutaMAX Supplement instead of L-glutamine. Addition of Pluronic F-68 is not required.

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When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-glutamine or GlutaMAX Supplement to Efficient-Pro Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then add anti-clumping agent (ACA) to your cultures at 1% v/v.

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Efficient-Pro Medium contains 4.7 g/L of glucose.

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Cells that are grown in or adapted to, and frozen in other cell culture media readily adapt to Efficient-Pro Medium. However, if your cells are experiencing a significant lag or delay in growth, please directly adapt the cells into Efficient-Pro Medium and passage them for a minimum of 3 passages, until a consistent doubling time is observed.

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