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Pierce™ Bradford Plus Protein Assay Kit with Dilution-Free™ BSA Protein Standards, multichannel pipette compatible, 950 mL - FAQs

Pierce™ Bradford Plus Protein Assay Kit with Dilution-Free™ BSA Protein Standards, multichannel pipette compatible, 950 mL - FAQs

View additional product information for Pierce™ Bradford Plus Protein Assay Kits - FAQs (A55866, 23236)

9 product FAQs found

What is the difference between the Pierce Dilution-Free BSA Protein Standards that come with the Pierce Bradford Plus Protein Assay Kit and the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209)?

Pierce Dilution-Free BSA Protein Standards are provided in a convenient 8-channel tubestrip. Each tube contains 1 mL of pre-diluted BSA protein standard at each concentration required for generating a standard curve. In the Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55866), the BSA concentrations included are 2000, 1500, 1000, 750, 500, 250, and 125 µg/mL. Each tubestrip contains one empty tube which allows the user to add their own blank solution for the purpose of background correction. This convenient format eliminates the time-consuming dilution step required for other single-concentration protein standard formats like the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209).

Additionally, the tubestrips are compatible with multichannel pipettes which allows the user to easily transfer the full standard curve dilution series (including blank) to a microplate simultaneously. This minimizes pipetting time associated with traditional protein standard preparation or pre-diluted protein standards which are typically packaged in 7 separate vials, requiring the user to open each vial and pipette each standard individually.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How does the Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible compare with the original Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236)?

The Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55866) includes the same assay reagent as the Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236), formerly called the Pierce Coomassie Plus (Bradford) Assay Kit. The only difference between the two products is the BSA protein standard format. While the Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236) includes the Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209), the Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible (Cat. No. A55866) includes BSA protein standards that are pre-diluted and packed in a convenient, multichannel pipette-friendly format. This format provides the following advantages:

-No protein standard dilution step needed
-High-throughput (multichannel pipette compatible)
-Blank tube provided for background correction
-Reduced hands-on assay time
-Reduced chances for pipetting errors

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can I purchase the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible included in the Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible as a standalone item?

Yes, the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, 0.125-2 mg/mL can be purchased separately (Cat. No. A55863).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

If we are assaying immunoglobin samples, do you offer bovine gamma globulin (BGG) protein standards in the Dilution-Free format?

We currently do not offer bovine gamma globulin (BGG) protein standards in the same multichannel pipette compatible format as the Pierce Dilution-Free BSA Protein Standards. They are only available as Pierce Bovine Gamma Globulin Standard Ampules, 2 mg/mL (Cat. No. 23212) and Pierce Bovine Gamma Globulin Standard Pre-Diluted Set (Cat. No. 23213).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Is the BSA in the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible the same as the BSA standards found in other Pierce protein assays and Pierce BSA protein standard products?

The BSA in the Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible included in the Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit (Cat. No. A55860, A55861, A55862) is identical to the BSA included in the Pierce Rapid Gold BCA Protein Assay Kit (Cat. No. A53225, A53226, A53227). It is also identical to the BSA found in Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209) and the Pierce Bovine Serum Albumin Standard Pre-Diluted Set (Cat. No. 23208).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How can I prevent contamination of the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible?

To ensure that the BSA standards remain free of contaminants, we recommend minimizing the time the tubestrips are uncovered/uncapped. Immediately place the capstrip over the tubes after each use. Be sure to press the caps firmly when closing so that it fits snugly. Store the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the orientation of the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible?

For Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible, the tubestrip has a single printed label on the top-center of the first tube indicating the tube containing the 2 mg/mL concentration (for Cat. No. A55863) or 10 mg/mL concentration (for Cat. No. A56979). Each subsequent tube contains the next highest concentration in the dilution series. The end-tube opposite the printed label is left empty to allow the user to add their own blank solution for the purpose of background correction. On the capstrip, there is a small hole at one end that can be used to orient the caps. When placing the caps on the tubestrip, we recommend aligning the hole-side of the capstrip with the labeled side of the tubestrip.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How do I open the Pierce Dilution-Free tubestrips?

To access the BSA standards, hold one side of the tubestrip firmly and carefully pull the foil seal to remove it from the tubestrip. The standards come with a capstrip for convenient closing and storage between uses.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the shelf-life of the Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible?

The Pierce Dilution-Free BSA Protein Standards, Multichannel Pipette Compatible are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment if stored as recommended. The product should be stored at 4 degrees C after opening. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.