Thermo Scientific™

POROS™ CaptureSelect™ FcXP Affinity Resin

Catalog number: A56254
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Thermo Scientific™

POROS™ CaptureSelect™ FcXP Affinity Resin

Catalog number: A56254
Have Questions?
Catalog Number
A56254
Unit Size
10,000 mL
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Availability
-
Quantity
-
Catalog Number
Unit Size
50 mL
Price (USD)
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Catalog Number
Unit Size
250 mL
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Catalog Number
Unit Size
1000 mL
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Catalog Number
Unit Size
5000 mL
Price (USD)
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-
Quantity
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Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A5625410,000 mL
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A5624950 mL
-
A56251250 mL
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A562521000 mL
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A562535000 mL
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POROS CaptureSelect FcXP Affinity Resin is specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma-derived IgG through binding of the CH3 domain of human IgG. The FcXP ligand recognizes all human IgG subclasses (IgG1, 2, 3, and 4) with high binding capacity (20–25 g IgG/L) and excellent elution characteristics. The large pore structure of the POROS bead enables efficient purification of larger biomolecules.

Features of FcXP affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• One-step purification from crude material with high purity and yield
• High specificity and capacity at higher flow rates
• Excellent scalability
• Non-animal derived

POROS CaptureSelect FcXP Affinity Resin is intended for the purification of IgG directly from plasma and Fc fusion proteins from other complex source materials. The resin enables a robust and efficient purification process with excellent purity obtained in a single step.

Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
Average particle size: 50 μm
Ligand: single-domain (VHH) antibody fragment
Binding capacity: 20–25 g IgG/L resin independent of flow rate, column height, and contact time
Elution conditions: 20 mM acetic acid, pH 4
Mechanical resistance: 100 bar (1,450 psi, 10 MPa)
Formulation buffer: 18% (v/v) ethanol

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Shipping Condition
Wet ice
Particle Size
50 μm
Form
Resin Slurry
Product Line
POROS™ CaptureSelect™
Quantity
10 L
Type
Resin

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