Protein oxidation, tyrosine nitration, and inactivation of sarcoplasmic reticulum Ca2+-ATPase in low-frequency stimulated rabbit muscle.
AuthorsKlebl BM, Ayoub AT, Pette D
JournalFEBS Lett
PubMed ID9498821
Sustained contractile activity by chronic low-frequency stimulation in rabbit fast-twitch muscle causes a partial (40-50%) inactivation of the sarcoplasmic reticulum (SR) Ca2+-ATPase and, with prolonged stimulation, a SERCA1a to SERCA2a transition. To investigate the underlying mechanism of the inactivation which precedes the isoform transition, we analyzed SR from 4-day stimulated ... More
Raft-partitioning of the ubiquitin ligases Cbl and Nedd4 upon IgE-triggered cell signaling.
AuthorsLafont F, Simons K
JournalProc Natl Acad Sci U S A
PubMed ID11248052
The high affinity receptor for IgE, FcepsilonRI on mast cells and basophils plays an essential role in immunological defense. Upon multivalent antigen binding, FcepsilonRI becomes phoshorylated by the protein-tyrosine kinase Lyn, as a result of receptor clustering in lipid rafts. FcepsilonRI has been shown to be ubiquitinated. Ubiquitination can lead ... More