SuperScript™ IV Template Switching RT Master Mix, 48 reactions - FAQs

View additional product information for SuperScript™ IV Template Switching RT Master Mix - FAQs (A65423, A65424, A65425)

6 product FAQs found

What template switching oligos can be used with SuperScript IV Template Switching Master Mix?

Invitrogen Template Switching Oligo (Cat. No. A65427) is offered, in conjunction with Capturing Oligo (dT) (Cat. No. A65429) and Preamplification Primers (Cat. No. A65428), complementary to the TSO. Alternatively, custom self-designed template switching oligo can be used.

Can SuperScript IV Template Switching RT Master Mix be used for other applications that do not involve template switching, e.g. RT-qPCR?

Yes, the master mix can be used for the first strand cDNA synthesis without templates switching oligo, but the results may be not as good as with more specialized product. For RT-qPCR, we recommend SuperScript IV VILO Master Mix.

SuperScript IV Template Swiching RT Master Mix is supplied with lysis buffer. Why does the protocol recommend using lysis buffer even with purified RNA samples?

Lysis buffer supplied with SuperScript IV Template Switching RT Master Mix has a dual function. It enables direct cDNA synthesis workflow without RNA purification, and it includes additional amount of proprietary enhancer which improves templates switching efficiency with low input samples of purified RNA (up to 10 ng).

What template switching oligos can be used with SuperScript IV Template Switching Master Mix?

Invitrogen Template Switching Oligo (A65427) is offered, in conjunction with Capturing Oligo (dT) (A65429) and Preamplification Primers (A65428), complementary to the TSO. Alternatively, custom self-designed template switching oligo can be used.

Can SuperScript IV Template Switching RT Master Mix be used for other applications that do not involve template switching, e.g. RT-qPCR?

Yes, the master mix can be used for the first strand cDNA synthesis without templates switching oligo, but the results may be not as good as with more specialized product. For RT-qPCR, we recommend SuperScript IV VILO Master Mix.

SuperScript IV Template Switching RT Master Mix is supplied with lysis buffer. Why does the protocol recommend using lysis buffer even with purified RNA samples?

Lysis buffer supplied with SuperScript IV Template Switching RT Master Mix has a dual function. It enables direct cDNA synthesis workflow without RNA purification, and it includes an additional amount of proprietary enhancer which improves templates switching efficiency with low input samples of purified RNA (up to 10 ng).