Covalent surface chemistry gradients for presenting bioactive peptides.
AuthorsKipper MJ, Kleinman HK, Wang FW
JournalAnal Biochem
PubMed ID17339030
'The activation of surfaces by covalent attachment of bioactive moieties is an important strategy for improving the performance of biomedical materials. Such techniques have also been used as tools to study cellular responses to particular chemistries of interest. The creation of gradients of covalently bound chemistries is a logical extension ... More
Surface adsorption and transfer of organomercaptans to colloidal gold and direct identification by matrix assisted laser desorption/ionization mass spectrometry.
AuthorsKirk JS, Bohn PW
JournalJ Am Chem Soc
PubMed ID15125684
'The adsorption characteristics of two different organomercaptan adsorbates, 5-((2-(and-3)-S-(acetylmercapto)succinoyl)amino)fluorescein (SAMSA) and the peptide Cys-Lys-Trp-Ala-Lys-Trp-Ala-Trp (CKWAKWAK), on colloidal Au were studied, and the conjugates produced were characterized by UV-vis spectroscopy, transmission electron microscopy, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Fluorescence difference measurements of free thiols in solution were used to ... More
Dual fluorescent labeling method to visualize plasmid DNA degradation.
'The efficiency of nonviral vectors for gene delivery may be enhanced by understanding the key barriers that limit the translocation of the therapeutic DNA into the nucleus. One such barrier is the instability of DNA in the cytoplasm. In this work, we have developed a method to dual-label plasmid DNA ... More
Tumor targeting with antibody-functionalized, radiolabeled carbon nanotubes.
'Single-walled carbon nanotubes (CNT) are mechanically robust graphene cylinders with a high aspect ratio that are comprised of sp(2)-bonded carbon atoms and possessing highly regular structures with defined periodicity. CNT exhibit unique mechanochemical properties that can be exploited for the development of novel drug delivery platforms. We hypothesized that novel ... More
Protective efficacy of CAP18106-138-immunoglobulin G in sepsis.
Naturally present antibacterial proteins play an important role in innate host defense. A synthetic peptide mimicking the C-terminal lipopolysaccharide (LPS)-binding domain of rabbit cathelicidin CAP18 was coupled to immunoglobulin (Ig) G to create CAP18(106-138)-IgG, a construct that, in concentrations equimolar to those of peptide alone, binds and neutralizes LPS and ... More
Design, characterization, and utilization of a fast fluorescence derivatization reaction utilizing o-phthaldialdehyde coupled with fluorescent thiols.
AuthorsHapuarachchi S, Aspinwall CA
JournalElectrophoresis
PubMed ID17311246
We have developed a chemical derivatization scheme for primary amines that couples the fast kinetic properties of o-phthaldialdehyde (OPA) with the photophysical properties of visible, high quantum yield, fluorescent dyes. In this reaction, OPA is used as a cross-linking reagent in the labeling reaction of primary amines in the presence ... More
Nanofluidic injection and heterogeneous kinetics of organomercaptan surface displacement reactions on colloidal gold in a microfluidic stream.
AuthorsKirk JS, Sweedler JV, Bohn PW
JournalAnal Chem
PubMed ID16579617
Colloidal gold is developed as a molecular capture reagent in hybrid nanofluidic-microfluidic devices for mass-limited sample analysis. Two fluorescent organomercaptans are injected through a nanocapillary array membrane and subsequently captured at the surface of 19-nm-diameter colloidal Au nanoparticles. The surface displacement kinetics are monitored via quenching of the organomercaptan fluorescence ... More
Conjugation of soluble CD4 without loss of biological activity via a novel carbohydrate-directed cross-linking reagent.
Chemical conjugates of recombinant soluble CD4 (sCD4) with toxins, or with antibodies that activate cytotoxic T cells, can be used to direct selective killing of human immunodeficiency virus (HIV)-infected cells. This approach takes advantage of the ability of sCD4 to bind with high affinity to gp120, the envelope protein of ... More