Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
AuthorsHiller Y, Gershoni JM, Bayer EA, Wilchek M
JournalBiochem J
PubMed ID3435435
A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. ... More
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
AuthorsHirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
JournalAnal Biochem
PubMed ID12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
AuthorsEvans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS
JournalProc Natl Acad Sci U S A
PubMed ID16263923
'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More
The structure of the complex between avidin and the dye, 2-(4'-hydroxyazobenzene) benzoic acid (HABA).
AuthorsLivnah O, Bayer EA, Wilchek M, Sussman JL
JournalFEBS Lett
PubMed ID8344421
'The crystal structure of the complex formed between the egg-white biotin-binding protein, avidin, and the dye, 2-(4''-hydroxyazobenzene) benzoic acid (HABA), was determined to a resolution of 2.5 A. The interaction of avidin with the benzoate ring of HABA is essentially identical to that of the complex formed between HABA and ... More
Three-dimensional structures of avidin and the avidin-biotin complex.
AuthorsLivnah O, Bayer EA, Wilchek M, Sussman JL
JournalProc Natl Acad Sci U S A
PubMed ID8506353
'The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight binding of biotin. Each ... More
Egg white avidin. 3. Sequence of the 78-residue middle cyanogen bromide peptide. Complete amino acid sequence of the protein subunit.
AuthorsDeLange RJ, Huang TS
JournalJ Biol Chem
PubMed ID5100763
Avidin.
AuthorsGreen NM
JournalAdv Protein Chem
PubMed ID237414
The use of the avidin-biotin complex as a tool in molecular biology.
AuthorsBayer EA, Wilchek M
JournalMethods Biochem Anal
PubMed ID7392958
The avidin-biotin complex in affinity cytochemistry.
AuthorsBayer EA, Skutelsky E, Wilchek M
JournalMethods Enzymol
PubMed ID440114
Prevention of nonspecific binding of avidin.
AuthorsDuhamel RC, Whitehead JS
JournalMethods Enzymol
PubMed ID2388571
BAC mapping using fluorescence in situ hybridization.
AuthorsChen XN, Korenberg JR
JournalMethods Mol Biol
PubMed ID15020820
COMBINABILITY OF AVIDIN AND STREPTAVIDIN WITH ANALOGS OF BIOTIN.
AuthorsLICHSTEIN HC, BIRNBAUM J
JournalBiochem Biophys Res Commun
PubMed ID14341938
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
AuthorsWood GS, Warnke R
JournalJ Histochem Cytochem
PubMed ID7028859
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed ... More
A sensitive enzyme assay for biotin, avidin, and streptavidin.
AuthorsBayer EA, Ben-Hur H, Wilchek M
JournalAnal Biochem
PubMed ID3706735
Reciprocal enzyme assays are described for the vitamin biotin and for the biotin-binding proteins avidin and streptavidin. The assays are based on the following steps: (a) biotinylated bovine serum albumin is adsorbed onto microtiter plates; (b) streptavidin (or avidin) is bound to the biotin-coated plates; (c) biotinylated enzyme (in this ... More
Dissociation rate constant of the biotin-streptavidin complex.
AuthorsPiran U, Riordan WJ
JournalJ Immunol Methods
PubMed ID2212686
We measured the dissociation rate constants of the biotin/streptavidin and biotin/egg avidin complexes by following the release of radiolabeled biotin from the preformed complexes in the presence of excess unlabeled biotin. For separation of bound and free labeled biotin we employed ultrafiltration with disposable microconcentrators. The dissociation rate constant for ... More
Grafting protein ligand monolayers onto the surface of microparticles for probing the accessibility of cell surface receptors.
AuthorsFrey A, Meckelein B, Schmidt MA
JournalBioconjug Chem
PubMed ID10411452
Coupling of a specific ligand to vaccines or drugs can be a powerful aid to route these compounds to a certain target cell population. However, if the targeted receptor is buried in a glycocalyx, binding of the ligand may be sterically hindered or even abolished, especially when the ligand is ... More
Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin.
AuthorsHiller Y, Bayer EA, Wilchek M
JournalBiochem J
PubMed ID1898347
The object of this study was to define minimized biotin-binding fragments, or 'prorecognition sites', of either the egg-white glycoprotein avidin or its bacterial analogue streptavidin. Because of the extreme stability to enzymic hydrolysis, fragments of avidin were prepared by chemical means and examined for their individual biotin-binding capacity. Treatment of ... More