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Verso cDNA Synthesis Kit, 40 reactions - FAQs

View additional product information for Verso cDNA Synthesis Kit - FAQs (AB1453A)

3 product FAQs found

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.

What is the size of cDNA that can be obtained from the Verso cDNA Synthesis Kit (Cat. No. AB1453B)

The Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42 degrees C to 57 degrees C. For improved performance, we recommend SuperScript IV Reverse Transcriptase.