Thermo Scientific™

Verso cDNA Synthesis Kit

Catalog number: AB1453A
Have Questions?
Thermo Scientific™

Verso cDNA Synthesis Kit

Catalog number: AB1453A
Have Questions?
Catalog Number
AB1453A
Unit Size
40 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AB1453A40 reactions
-
AB1453B100 reactions
-
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The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Features of the Verso cDNA Synthesis Kit include:
• Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR

Applications
• 2-step RT-(q)PCR
• Single stranded cDNA synthesis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Reaction Speed
30 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
Verso
Ribonuclease H Activity
Yes
Shipping Condition
Wet or Dry Ice
For Use With (Application)
Real Time PCR (qPCR), RT-PCR
Final Product Type
First-Strand cDNA
No. of Reactions
40 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 11 kb
Starting Material
RNA

Contents & Storage

• RT Enzyme mix with RNase Inhibitor
• 5X RT Buffer
• Random Hexamers
• Anchored Oligo (dT)
• 20 mM dNTPs
• RT Enhancer

Store at –20°C.

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