Verso cDNA Synthesis Kit, 100 reactions - FAQs

View additional product information for Verso cDNA Synthesis Kit - FAQs (AB1453B)

5 product FAQs found

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.

What is the error rate of the Verso reverse transcription enzyme (Cat. No. AB1453A, AB1453B)?

Error rate is approximately 1 error per 15-27x 10^3 bases.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the size of cDNA that can be obtained from the Verso cDNA Synthesis Kit (Cat. No. AB1453B)

The Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42 degrees C to 57 degrees C. For improved performance, we recommend SuperScript IV Reverse Transcriptase.

What are the features of the Verso cDNA Synthesis Kit (Cat. No. AB1453A, AB1453B)?

The Verso cDNA synthesis Kit enables robust transcription of RNA to create a complete cDNA pool. The Verso system allows robust and sensitive reverse transcription through the combination of a high-affinity RT enzyme, a trusted RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.