Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Catalog number: AB1454LDA

Thermo Scientific™  Related applications: Reverse Transcription

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Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix (with ThermoPrime Taq) is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to corresponding increases in product generated over a wider range of starting material concentrations.

An optional RT Enhancer is provided with the kit and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment.

This Verso 1-step RT-PCR system comes with the ReddyMix option, where the master mix contains a dye and precipitant that allow RT-PCR reactions to be loaded directly onto an electrophoresis gel. The red dye migrates between bromophenol blue and xylene cyanol at approximately 300 bp, depending on agarose concentration.


• ReddyMix formulation—eliminates post-PCR steps required for electrophoresis
• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and PCR steps performed in single sealed tube—convenience and reduced contamination risk
• Verso enzyme is highly sensitive in a broad dynamic range—accurately detect RNA input from 1 pg to 1 µg
• Wide working temperature range (42 to 57°C) for success with GC-rich and other difficult templates
• RNase Inhibitor included in RT enzyme mix

• Gene expression analysis
• Viral/bacterial detection
• 1-step RT-PCR
• High throughput applications

Related Products
Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq
Verso 1-Step RT-PCR Kit, with Thermo-Start Taq (Hot Start)
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step RT-PCR
Reverse Transcriptase: Verso Reverse Transcriptase
Polymerase: ThermoPrime Taq DNA Polymerase
Hot Start: No
Sample Type (General): RNA
Final Product Size(s): 3 kb or less
Product Line: Verso
Product Size: 40 reactions

Contents & storage

• Verso Enzyme Mix (includes RNase inhibitor)
• 2X Master Mix (ThermoPrime Taq DNA Polymerase, RT buffer, dNTPs, MgCl2 in ReddyMix formulation)
• RT Enhancer

Store at -20°C.


Manuals & protocols