Verso 1-Step RT-PCR Kit, with Thermo-Start Taq (Hot Start)

Catalog number: AB1455B

Thermo Scientific™  Related applications: Reverse Transcription

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Thermo Scientific Verso 1-Step RT-PCR Kit with Thermo-Start Taq (Hot Start) is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to corresponding increases in product generated over a wider range of starting material concentrations.

Thermo-Start Taq DNA Polymerase is a chemically-modified polymerase that remains inactive throughout the RT step, activated only by the high-temperature incubation (95°C for 15 minutes) prior to PCR. It delivers high yields and greatly reduces nonspecific primer extension before PCR.

An optional RT Enhancer is provided with kits and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment.


• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and PCR steps performed in single sealed tube—convenience and reduced contamination risk
• Verso enzyme is highly sensitive in a broad dynamic range—accurately detect RNA input from 1 pg to 1 µg
• Wide working temperature range (42 to 57°C) for success with GC-rich and other difficult templates
• RNase Inhibitor included in RT enzyme mix


• Gene expression analysis
• Viral/bacterial detection
• 1-step RT-PCR
• High throughput applications

Related Products
Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq
Verso 1-Step RT-PCR Kit, with Thermo-Start Taq (Hot Start)
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Reverse Transcriptase: Verso Reverse Transcriptase
Polymerase: Thermo-Start Taq DNA Polymerase
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Final Product Size(s): 3 kb or less
Product Line: Verso
Product Size: 200 reactions

Contents & storage


• Verso Enzyme Mix (includes RNase inhibitor)
• 2X Master Mix (ThermoStart Taq DNA Polymerase, RT buffer, PCR buffer, dNTPs, MgCl2)
• RT Enhancer
Store at -20°C.


Manuals & protocols