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Verso 1-step RT-qPCR Kit, SYBR Green, low ROX, 200 reactions - FAQs

Verso 1-step RT-qPCR Kit, SYBR Green, low ROX, 200 reactions - FAQs

View additional product information for Verso 1-step RT-qPCR Kit, SYBR Green, low ROX - FAQs (AB4106A, AB4106C)

3 product FAQs found

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.

Can I purchase the Verso Enzyme Mix separately from the Verso 1-step RT-qPCR Kit (Cat. No. AB4016A, AB4016C)?

No, we do not offer the Verso Enzyme Mix as a standalone product. It is only available as a component of the Verso 1-step RT-qPCR Kit (Cat. No. AB4016A, AB4016C).