Invitrogen™

MAXIscript™ SP6 Kit with Manual

Catalog number: AM1308M
Invitrogen™

MAXIscript™ SP6 Kit with Manual

Catalog number: AM1308M
Catalog Number
AM1308M
Unit Size
30 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
30 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM1308M30 reactions
-
AM1310M100 reactions
-
AM1310100 reactions
-
AM130830 reactions
-
Product Overview
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The Ambion™ MAXIscript™ SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 100 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript™ In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript™ Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript™ Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript™ Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 μg/20 μl reaction) of up to 2 kb.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type
Synthetic mRNA, mRNA
Format
Kit
Sample Type
mRNA, Synthetic mRNA
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions
30 Reactions
Product Line
Ambion™, MAXIscript™
Product Type
SP6 In Vitro Transcription kit
Quantity
30 reactions
Promoter
SP6
Yield
2-6 μg
Starting Material
PCR Products, Linearized DNA plasmid

Contents & Storage

SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

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