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MAXIscript™ SP6/T7 Transcription Kit
The Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protectionRead more
| Catalog Number | Product Type | Quantity |
|---|---|---|
| AM1322 | SP6/T7 In Vitro Transcription kit | 100 Reactions |
Catalog number AM1322
Price (USD)
418.00
Each
Product Type:
SP6/T7 In Vitro Transcription kit
Quantity:
100 Reactions
Price (USD)
418.00
Each
The Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 50 reactions of each enzyme.
• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents
The MAXIscript™ In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript™ Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript™ Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript™ Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 μg/20 μl reaction) of up to 2 kb.
• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents
The MAXIscript™ In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript™ Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript™ Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript™ Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 μg/20 μl reaction) of up to 2 kb.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions100 Reactions
Product LineAmbion, MAXIscript
Product TypeSP6/T7 In Vitro Transcription kit
PromoterT7, SP6
Quantity100 Reactions
Shipping ConditionDry Ice
Starting MaterialPCR Products, Linearized DNA plasmid
Yield2-6 μg
Final Product TypeSynthetic mRNA, mRNA
FormatKit
Sample TypemRNA, Synthetic mRNA
Unit SizeEach
Contents & Storage
SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.