BrightStar® Psoralen-Biotin Nonisotopic Labeling Kit with manual - FAQs

1. Sample purity:

- There should be no free nucleotides in the preparation (e.g., from PCR).
- RNA samples must be free of DNA, e.g. a transcription template.
- Samples must be free of proteins.
- Nucleic acid solutions should be free of phenol or alcohol contaminants, the pH should be between 2.5 and 10, and the salt concentration should be less than 20 mM.

2. The sample concentration should be 0.5-50 ng/µL.

It can be used to label single-stranded antisense RNA for northerns, Southerns, or in situ hybridization. The same kit can also be used to label PCR products, plasmids, gel-purified cDNA inserts, and oligonucleotides for northern and Southern blotting and in situ hybridization. Sensitivity is comparable to that obtained with 32P-radiolabeled probes and enzymatically synthesized biotin-labeled probes when used with the BrightStar BioDetect Kit for nonisotopic detection and BrightStar-Plus Positively Charged Nylon Membranes.

These probes are not subject to radiolysis like radiolabeled probes; therefore, you can store them for at least one year.