mirVana™ miRNA Probe Construction Kit

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The Ambion® mirVana™ miRNA Probe Construction Kit uses in vitro transcription to generate short (<100 nt) radiolabeled or nonisotopically labeled RNA probes in less than 1 hour. The mirVana™ miRNA Probe Construction Kit includes reagents for preparing 30 in vitro transcription templates as well as reagents for 30 in vitro transcription reactions.

• Rapid procedure—prepare transcription templates and synthesize RNA probes in less than 1 hour
• Ideal for siRNA/miRNA studies
• Compatible with the mirVana™ miRNA Detection Kit, northern analysis, and in situ hybridization
• No cloning required
• More economical than labeling chemically synthesized RNA
• Produces probes with higher specific activity than 5' end labeling, resulting in greater sensitivity.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Biotin-Based, Fluorescent, Radioactive
Final Product: Probes (Labeled RNA)
Includes Label or Dye: No
Label or Dye: 32P (Phosphorous-32), Biotin
Labeling Method: Direct Labeling
Labeling Target: RNA (General)
Product Line: mirVana™, Ambion™
Product Size: 30 reactions
Shipping Condition: Dry Ice

Contents & storage

DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP Mix, Exo– Klenow, T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, Control DNA Primer, DNase I, Gel Loading Buffer II, and Probe Elution Buffer are stored at –20°C. Nuclease-free Water may be stored at any temperature.


Manuals & protocols