Webinar: "Sensitive detection and analysis of microRNAs in limited biofluid samples"—register now.

mirVana™ PARIS™ RNA and Native Protein Purification Kit

Catalog number: AM1556

Ambion™  Related applications: Protein Purification & Isolation | RNA Extraction | RNAi | miRNA & Non-Coding RNA Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The mirVana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample. Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions. Features of this kit:

• Isolate small RNA-containing total RNA and native protein from the same sample
• Start with 100–107 cultured cells or up to 100 mg of many mammalian tissues
• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses
• Follow a simple, 30-minute procedure
• Use to correlate mRNA, miRNA or siRNA, and protein levels

How the kit works
The mirVana™ PARIS™ Kit procedure begins with homogenization of samples with a special cell disruption buffer that includes nonionic detergent. Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications. The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both.

Accessory products
For tissues that have very high levels of ribonucleases, such as pancreas or spleen, the mirVana™ miRNA Isolation Kit is recommended, although it is not designed to recover protein and small RNA from the same sample.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Real-Time Quantitative PCR (qPCR), microRNA Analysis, Western Blotting, cDNA Library Construction
Product Size: 40 preps
Final Product: Total RNA, siRNA, snRNA, Protein (Native), Transcriptome RNA, micro RNA
Isolation Technology: Organic Extraction, Spin Column (Glass Fiber Filter), Cell Lysis (Nonionic Detergent)
Sample Type (Specific): Cell Cultures, Tissue (Mammalian)
Starting Material (Amount): Up to 100 mg tissue, Up to 10^7 cells
Shipping Condition: Room Temperature

Contents & storage

• 30 ml miRNA Wash Solution I (room temperature)
• 50 ml Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 25 ml Cell Disruption Buffer (4°C)
• 25 ml 2X Denaturing Solution (4°C)
• 2 × 25 ml Acid-Phenol:Chloroform (4°C)
• 5 ml Elution Solution (-20°C, 4°C, or room temperature)

Documents

Manuals & protocols

Recommended products