mirVana™ miRNA Isolation Kit, without phenol - FAQs

View additional product information for mirVana™ miRNA Isolation Kit, without phenol - FAQs (AM1561)

3 product FAQs found

What are different methods for cleaning up my miRNA sample?

- Precipitation of miRNA: Follow the instructions for the mirVANA miRNA Isolation Kit or the mirVANA PARIS RNA and Native Protein Purification Kit.
- Double phenol/guanidium (chaotropic reagent) extraction: Disrupt tissue with phenol/guanidine, then extract with phenol, centrifuge, remove the supernatant, and precipitate the RNA.
- Glass-fiber filter: Disrupt the tissue in a chaotropic agent, bind the RNA to a glass-fiber filter, then wash off unbound DNA/protein/salt/nucleotides and elute the RNA.

Do you offer a stand-alone phenol product that I can use with mirVana miRNA Isolation Kit, without phenol (Cat. No. AM1561)?

Yes, we offer Acid-Phenol: Chloroform, pH 4.5 (with IAA, 125:24:1), Cat. No. AM9720 or AM9722.
Note: We also offer mirVana miRNA Isolation Kit, with phenol (Cat. No. AM1560) that already comes with phenol.

What kit do you recommend to capture small RNA that is >17 nt in size?

We recommend using the mirVana miRNA Isolation Kit (Cat. No. AM1560, AM1561), both of which are designed to capture small RNAs, such as miRNAs, tRNAs, etc. Purification of RNAs as small as 20 nt has been obtained in-house using these kits. Note: Cat. No. AM1561 does not contain phenol, but is required for the procedure. Phenol can be purchased separately (Cat. No. AM9720).