Silencer® siRNA Labeling Kit with FAM dye

Catalog number: AM1634

Ambion™  Related applications: RNAi

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Description

For the labeling of siRNA with FAM™. Includes sufficient reagents for labeling 65 µg of siRNA. Ambion® Labeled siRNA can be used to analyze siRNA subcellular localization, stability, and transfection efficiency. In addition, fluorescently labeled siRNA is particularly well-suited for use in double-label experiments (with a labeled antibody) to track cells that receive siRNA during transfection and to correlate transfection with down-regulation of the target protein. Research shows that labeling of siRNAs does not affect their biological function.

Accessory Products:
Also available is the Silencer® Cy™3 siRNA Labeling Kit (SKU# AM1632).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: FITC (Fluorescein)
Product Size: 1 kit
Final Product: siRNA (Labeled)
Labeling Method: Direct Labeling
Labeling Target: siRNA
Detection Method: Fluorescent
Includes Label or Dye: Yes
Shipping Condition: Dry Ice

Contents & storage

10X Labeling Buffer, Reconstitution Solution, 5M NaCl, 5X siRNA Annealing Buffer, GAPDH siRNA, and FAM™ Labeling Reagent should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Documents

Manuals & protocols