I am using the KDalert GAPDH Assay Kit and am not seeing any GAPDH activity in my standard curve dilutions. What is the problem?
If none of the standard curve samples turn a magenta color after 15 minutes, there was most likely an error in preparing the KDalert Master Mix. We suggest repeating the GAPDH Enzyme dilution standard curve reactions.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.