Where can I find more information about published miRNA sequences and annotation?
Please visit miRBase: The microRNA database (http://www.mirbase.org/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
I would like to order a mimic or inhibitor with a 5' label. Can you do this?
We can label our Pre-miR and Anti-miR miRNA, but do not offer labeled mirVana Mimics and inhibitors. 5' modifications include Alexa Fluor 555, Alexa Fluor 647, FAM, and Cy3. Request a quote here (http://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
The miRNA sequence I need to target is not in the miRBase database. Can I order a custom mimic or inhibitor?
Yes, submit your unpublished miRNA sequences and/or order custom modified miRNA here (http://www.thermofisher.com/order/custom-genomic-products/tools/mirna/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
What is the difference between Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors and mirVana miRNA mimics and inhibitors?
Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors are our first generation mimic and inhibitors. Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation. Pre-designed inhibitors have been created for all miRNAs listed in miRBase version 15.0.
Invitrogen Pre-miR miRNA Precursors are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Please note, Pre-miR miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
mirVana miRNA mimics and inhibitors are our second generation of mimics and inhibitors. mirVana miRNA mimics are small, chemically double-stranded RNAs that mimic endogenous mature miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. They have enhanced specificity over the first generation mimics, are delivered as HPLC purified, and ready to transfect.
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. They have enhanced potency over our first generation inhibitors. Pre-designed inhibitors were created for all miRNAs listed in miRBase version 19.0. They come delivered as HPLC purified, and ready to transfect. Learn more about mirVana miRNA mimics and inhibitors through this video (https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw). Both mimics and inhibitors are available individually or as libraries.
They are compatible with both in vitro and in vivo applications, and have been validated with Lipofectamine RNAiMAX Transfection Reagent for use in cell-based systems, and with Invivofectamine 3.0 Transfection Reagent for in vivo delivery. These oligonucleotides are nontoxic and do not induce an immune response in the animal models tested. In vivo-ready mirVana miRNA mimics and inhibitors have been purified by HPLC and dialysis and are ready for immediate in vivo use. For all library inquiries, please email: rnailibraries@lifetech.com.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
What is the difference between siRNA and miRNA?
siRNA mediates degradation of a target mRNA molecule in a sequence-specific manner, while miRNA suppresses translation without cleaving mRNA.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
