QuantumRNA™ β-Actin Internal Standards

Catalog number: AM1720

Ambion™

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Description

The Ambion® QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimer™s to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed "housekeeping" gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Species: Mouse
Includes: Control Primers & Template(s)
Gene Symbol: ACTB
Target Gene: β-actin
Label or Dye: Unconjugated
Product Size: 100 reactions
Control Template: Total RNA from Mouse Liver
Shipping Condition: Dry Ice

Contents & storage

β-Actin Primer Pair and β-Actin Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.

Documents

Manuals & protocols