The Ambion® QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimer™s to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed "housekeeping" gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp
For Research Use Only. Not for use in diagnostic procedures.
Total RNA from Mouse Liver
Control Primers & Template(s)
Label or Dye
No. of Reactions
Size (Final Product)
Contents & Storage
β-Actin Primer Pair and β-Actin Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.
Relative RT-PCR Using Beta-actin Internal Standards
Why do I need to be in linear range when analyzing 2 products in relative RT-PCR?