This product will be discontinued on September 1, 2016. For superior performance, we recommend you switch to the SSIV 1st Strand Synthesis System.

MessageSensor™ RT Kit

Catalog number: AM1745

Invitrogen™  Related applications: Reverse Transcription

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Description

For the reverse transcription of RNA. The Ambion® kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions.
• Linear quantitation of mRNA targets over 106-fold range of input RNA
• Quantitate transcripts from as little as 500 fg total RNA—1⁄20th of a single cell
• Fast, flexible RT step complete in 15 min; use reaction temperatures up to 50°C
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods 
MessageSensor™ is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity (see figure). The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than 2 hours.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: M-MLV Reverse Transcriptase
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format: Separate Components
Sample Type (General): RNA
Product Size: 50 reactions
Final Product: cDNA
Shipping Condition: Dry Ice
Final Product Size(s): 7 kb or less
Sample Type (Specific): mRNA
Optimal Reaction Temperature: 42-50° C

Contents & storage

10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, and hGAPDH Control Primers should be stored at –20°C. HeLa S3 Control RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.

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