Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit

Catalog number: AM1821

Ambion™  Related applications: Microarray Analysis

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The Ambion® Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit includes reagents for high- throughput aRNA amplification with amimo allyl NTP incorporation. The kit includes sufficient reagents for 100 reactions; Cy™3 dye is not included.

• Consistently higher yields with magnetic bead- compared to filter-based purification
• Highly reproducible aRNA yields and size (CV less than 10%)
• Flexible format allowing RNA amplification with a partial or full 96-well plate
• Simple integration into robotic platforms

Improved First- and Second-strand cDNA Synthesis
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript™ RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.

Increased Labeling Efficiency of Your aRNA
The Amino Allyl MessageAmp™ aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy™ or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.

Accessory Product
The 96-Well Magnetic-Ring Stand (SKU#AM10050) features 96 powerful ring-shaped magnets arranged to cradle each well of a 96-well plate to capture beads thoroughly and quickly. Captured magnetic beads will form a donut-shaped pellet with a large hole in the center. This capture pattern facilitates both supernatant removal and subsequent bead resuspension. The stand is suitable for high-throughput applications conducted manually with multichannel pipettors or with robotic liquid handlers.
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 100 reactions
Labeling Method: Indirect Labeling
Includes Label or Dye: No
Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
Sample Type (Specific): Total RNA, Poly(A+) RNA

Contents & storage

The Amino Allyl MessageAmp™ II-96 aRNA Amplification Kit contains T7 Oligo(dT) Primer, ArrayScript™ RT, RNase Inhibitor, 10X First-Strand Buffer, dNTP Mix, 10X Second-Strand Buffer, DNA Polymerase, RNase H, T7 Enzyme Mix, T7 10X Reaction Buffer, T7 UTP Solution, aaUTP Solution, ATP⁄CTP⁄GTP Mix, Coupling Buffer, DMSO, Hydroxylamine and Control RNA (1 mg⁄mL HeLa total RNA) which are all stored at –20°C. cDNAPure and RNA Binding Beads are stored at +4°C. cDNA Wash Buffer Concentrate, aRNA Wash Solution Concentrate, Bead Resuspension Solution, aRNA Elution Buffer, aRNA Binding Buffer Concentrate, U-Bottom Plate, PCR Plate, Reservoir, Nuclease-free Water, and detailed Instruction Manual are stored at room temperature.


Manuals & protocols