DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Features of this reagent set include:
• Safely eliminate DNA contamination from RNA samples • No organic extraction or heat inactivation required • Includes novel reagent to remove DNase • Recombinant DNase I is certified RNase-free
Inactivation and removal of DNase DNA-free™ reagents effectively remove DNase and divalent cations from the reaction mixture. The DNase/cation removal step takes only three minutes. No organic extraction, EDTA addition, or heat inactivation is required. The DNA-free™ Kit comes complete with RNase-free DNase I, an optimized 10X Reaction Buffer, and a novel DNase Removal Reagent.
Accessory product TURBO DNA-free™ Kit (Cat. No. AM1907) is similar to the DNA-free™ Kit but includes TURBO™ DNase, an engineered hyperactive DNase that exhibits up to 350% greater catalytic efficiency than wild type DNase. The enzyme also has a 6-fold lower Km for DNA, thus enabling effective removal of trace quantities of DNA contamination.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Shipping Condition
Dry Ice
Enzyme
DNase
Compatible Buffer
10X Reaction Buffer
Product Type
DNA Removal Kit
Product Line
Ambion™, DNA-free™
Contents & Storage
Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.