Invitrogen™

MEGAclear™ Transcription Clean-Up Kit

Catalog number: AM1908
Invitrogen™

MEGAclear™ Transcription Clean-Up Kit

Catalog number: AM1908
Catalog Number
AM1908
Unit Size
20 preps
Price (USD)
152.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM190820 preps
152.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

Specifications

Final Product Type
purified synthetic mRNA, purified mRNA
Format
Tube
Isolation Technology
Silica Spin Column
Purification Target
mRNA, synthetic mRNA
Sample Type
mRNA, synthetic mRNA
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
For Use With (Equipment)
Vacuum Manifold, Centrifuge
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
20 Preps
Product Line
Ambion™, MEGAclear™
Product Type
mRNA purification kit, Purification kit
Quantity
20 Preps
Purification Time
30 min
Shipping Condition
Room Temperature
Starting Material Volume
1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Yield
500 µg

Contents & Storage

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.

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