RNAqueous®-Midi Total RNA Isolation Kit

Catalog number: AM1911

Ambion™  Related applications: RNA Extraction

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Northern Blotting, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Product Size: 15 preps
Final Product: Total RNA
Isolation Technology: Syringe Filter (Glass Fiber)
Shipping Condition: Room Temperature

Contents & storage


Formaldehyde Load Dye should be stored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, Lithium Chloride Solution, Wash Solution #1 and Wash Solution 2/3 Concentrate should be stored at 4°C; Syringe Filter Units, Collection Tubes and Elution Solution can be stored at room temperature. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

Recommended products