RNAqueous® Total RNA Isolation Kit, with Manual

Catalog number: AM1912M

Ambion™  Related applications: RNA Extraction

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The RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or from 107–108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Bacteria, Yeast, Cells, Plant Samples, Viral Samples, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Nuclease Protection Assays, Northern Blotting, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Real-Time Quantitative PCR (qPCR)
Product Size: 50 preps
Final Product: Total RNA
Isolation Technology: Spin Column (Glass Fiber Filter)

Contents & storage

Formaldehyde Load Dye should be stored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, LithiumChloride Solution, Wash Solution #1 and Wash Solution 2⁄3 Concentrate should be stored at 4°C; Filter Cartridges, Collection Tubes and Elution Solution can be stored at room temperature. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols