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RiboPure™ RNA Purification Kit, yeast

Catalog number: AM1926

RiboPure™ RNA Purification Kit, yeast

Catalog number: AM1926
Catalog Number
Unit Size
50 preps
Price (USD)
Price: 608.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM192650 preps
Price: 608.00
Your Price:
Product Overview
Citations & References
Additional Information
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion™ kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents

It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).

Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


Isolation Technology
Spin Column (Glass Fiber Filter)
Purification Time
1.5 hr. (Including DNase treatment)
Elution Volume
50–100 μL
Sample Type
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
50 Preps
Shipping Condition
Box 1: Dry Ice
Box 2: Room Temperature
Starting Material Amount
Up to 108 cells
Up to 300 μg

Contents & Storage

• 50 1.5-mL Screw Cap Tubes; store at room temperature
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes (2 mL); room temperature
• 24 mL Lysis Buffer; 4°C
• 2.4 mL 10% SDS; 4°C
• 24 mL Phenol:Chloroform:IAA; 4°C
• 95 mL Binding Buffer; 4°C
• 35 mL Wash Solution 1; 4°C
• 50 mL Wash Solution 2/3 (concentrated); 4°C
• 5 mL Elution Solution; 4°C
• 80 g Zirconia Beads; -20°C
• 550 µL 10X DNase 1 Buffer; -20°C
• 200 µL DNase 1; -20°C
• 550 µL DNase Inactivation Reagent; -20°C


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