Mouse RiboPure™-Blood RNA Isolation Kit

Catalog number: AM1951

Ambion™  Related applications: RNA Extraction

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Description

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion® kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater®, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater® Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Microarray Analysis, Real-Time Quantitative PCR (qPCR)
Species: Mouse
Product Size: 25 preps
Final Product: Total RNA, micro RNA
Yield (up to): 4 µg
Isolation Technology: Spin Column, Organic Extraction
Starting Material (Amount): Up to 0.5 ml whole blood
Shipping Condition: Room Temperature

Contents & storage

Acid–Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2⁄3 Concentrate at 4°C. Store 2 mL tubes pre–loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease–free Water and Elution solution at room temperature.

Documents

Manuals & protocols

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