This product will be discontinued on September 1, 2016. We recommend you switch to T4 RNA Ligase (10 U/µL).

T4 RNA Ligase (cloned) 5 U/μL

Catalog number: AM2140

Invitrogen™  Related applications: Nucleic Acid Labeling & Oligo Synthesis

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Description

Ambion® T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 500 U (5 U/ µL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Unit Definition:
One unit catalyzes the formation of 1 nmol of [5'-32P]-rA12-18 into a phosphatase-resistant form in 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: Ligase
Product Size: 500 units
Shipping Condition: Dry Ice

Contents & storage

T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.

Documents

Manuals & protocols

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