Invitrogen™

siPORT™ NeoFX™ Transfection Agent with manual

Catalog number: AM4511M
Invitrogen™

siPORT™ NeoFX™ Transfection Agent with manual

Catalog number: AM4511M
Catalog Number
AM4511M
Unit Size
1 mL
Price (USD)
582.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
400 µL
Price (USD)
343.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
400 µL
Price (USD)
332.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
620.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM4511M1 mL
582.00
Your Price:
***
AM4510400 µL
343.00
Your Price:
***
AM4510M400 µL
332.00
Your Price:
***
AM45111 mL
620.00
Your Price:
***
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
Recommendations
This Ambion™ lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 1 mL.

• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day

Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named “neofection”, will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.

Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.

Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).

Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer™ siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

Specifications

For Use With (Application)
Transfection
High-throughput Compatibility
High-throughput Compatible
Product Line
Ambion™, siPORT™
Product Type
Transfection Reagent
Quantity
1 mL
Serum Compatible
Yes
Cell Type
Stem Cell, Primary Cell, Cell Lines
Format
6-Well Plate, 12-Well Plate, 24-Well Plate, 48-Well Plate, 96-Well Plate, Flasks
Sample Type
Synthetic siRNA
Transfection Technique
Lipid-Based Transfection

Contents & Storage

Store at 4°C.

Figures