Invitrogen™

Silencer™ Negative Control No. 2 siRNA

Catalog number: AM4637
Invitrogen™

Silencer™ Negative Control No. 2 siRNA

Catalog number: AM4637
Catalog Number
AM4637
Unit Size
40 nmol
Price (USD)
528.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 nmol
Price (USD)
180.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM463740 nmol
528.00
Your Price:
***
AM46135 nmol
180.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Ambion™ Silencer™ Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the “hit” threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer™ siRNA Screening Control Panel (SKU#AM4640)
The Silencer™ siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer™ siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Specifications

Control Type
Negative Control
RNAi Type
siRNA
Species
Human, Mouse, Rat
For Use With (Application)
Transfection, RNAi
Label or Dye
Unconjugated
Product Line
Silencer™, Ambion™
Product Type
siRNA
Purity
HPLC
Quantity
40 nmol
Shipping Condition
Room Temperature

Contents & Storage

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

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