Promyelocytic Leukemia (HL-60) Total RNA

Catalog number: AM7836

Ambion™  Related applications: RNA Extraction

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Description

The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR. Note: While it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant. The integrity of the RNA is verified by capillary electrophoresis using the Agilent® 2100 bioanalyzer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7300 System, 7700 System, 7500 Fast System, 7900HT Fast System, Bio-Rad CFX Connect™, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, StepOnePlus™ System, 7500 Fast Dx System, Roche LightCycler 480, Bio-Rad CFX384 Touch™, ViiA™ 7 System
Reaction Speed: Fast or Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: Dual-Lock™ Taq DNA Polymerase
Product Size: 2 x 1 mL
Shipping Condition: Wet Ice
Number of Reactions: 200 reactions

Contents & storage

Store at or below -70°C. Avoid multiple freeze–thaw cycles. Aliquots of the product may be stored short-term at -20°C. DO NOT STORE IN FROST-FREE FREEZER.

Documents

Manuals & protocols