Invitrogen™

Cap Analog (m7G(5')ppp(5')G), 100 A254 Units

Catalog number: AM8052
Invitrogen™

Cap Analog (m7G(5')ppp(5')G), 100 A254 Units

Catalog number: AM8052
Catalog Number
AM8052
Unit Size
1 tube
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM80521 tube
-
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Ambion™ Cap Analog [m7G(5')ppp(5')G] is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. These molecules are translated more efficiently in in vitro translation systems than uncapped mRNAs. 100 A254 U supplied. Substitution of cap analog for a portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a corresponding fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. It is important that in vitro transcripts be capped for microinjection experiments because uncapped mRNAs are rapidly degraded. Cap analog is also used as a highly specific inhibitor of the initiation step of protein synthesis.

Quality Control:
Each Cap Analog is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Each is also assayed for the efficient synthesis of capped in vitro transcripts by using the cap analog in functional MEGAscript™ In Vitro transcription and Retic Lysate translation reactions.

Accessory Products:
Complete kits are available with all the necessary reagents for the in vitro transcription of capped RNA transcripts. The mMESSAGE mMACHINE™ T7 Ultra Kit includes ARCA (SKU #AM1345). Also available are kits containing conventional cap analog; mMESSAGE mMACHINE™ T7 (SKU #AM1344), T3 (SKU #AM1348), and SP6 (SKU #AM1340) Kits. The Retic Lysate IVT™ (SKU #AM1200) for in vitro translation of mRNA is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
40 mM
Final Product Type
Cap Analog for In vitro Transcription, Cap Analog for mRNA
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA
For Use With (Equipment)
mMessage mMAchine, MEGAscript
Labeling Method
RNA Labeling
Product Line
Ambion™
Product Type
Cap analog for mRNA, cap analog for synthetic mRNA
Quantity
100 A254 U
Shipping Condition
Dry Ice

Contents & Storage

Supplied in a single tube to be stored at or below –70°C. Avoid multiple freeze-thaw cycles. Aliquots of the product may be stored short-term at –20°C.

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