RNA Fragmentation Reagents

Catalog number:  AM8740

 Related applications:

miRNA & Non-Coding RNA Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


For the fragmentation of aRNA prior to hybridization with oligonucleotide arrays. The protocol is simple, fast and can be completed in less than 20 min. Sufficient Ambion® reagents are included for 200 rxns. Many protocols for using amplified RNA in microarray analysis recommend fragmenting the aRNA prior to hybridization with an oligonucleotide microarray. This fragmentation step improves hybridization kinetics with the arrayed oligonucleotides and can lead to enhanced signal. The protocol is simple. Mix the aRNA with the Ambion® 10X RNA Fragmentation Reagent and heat to 70°C for 15 min. The rxn is then stopped by the addition of the Stop Solution and placed on ice until ready for hybridization with the microarray. The average size of the resulting fragments will be 60-200 nucleotides. Up to 10 µg of RNA can be fragmented in a 10 µL rxn.
For Research Use Only. Not for use in diagnostic procedures.


Concentrated: 10 X
Form: Liquid
Product Line: Ambion™
Product Size: 200 reactions
Shipping Condition: Room Temperature

Contents & storage

10X Fragmentation Reagent and Stop Solution should be stored at room temperature.


Manuals & protocols