Formamide (Deionized), 5 x 500 g - FAQs

For long-term storage, we recommend resuspending the RNA in stabilized formamide and storing at -70 degrees C. To remove the formamide, add 4 volumes ethanol and, if less than 20 µg RNA, also add NaCl to 0.2 M. Precipitate the RNA and use for downstream experiments.

There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.